The Role of Bronchoalveolar Lavage in Diagnosing Nonopportunistic Bacterial Pneumonia (28)

In: Pulmonary function

20 May 2013

It can be easily combined with rapid identification methods such as DNA probes to diagnose tuberculosis earlier in patients. The use of alternative methods for direct nonculture-dependent detection of mycobacterial components such as nucleic acid hybridization, immunoassay for antigens, antibodies, or other markers has not as yet been applied to BAL samples in a routine clinical setting. Buy Advair Diskus Online
Mycoplasma pneumoniae
The laboratory diagnosis of mycoplasmal disease is generally retrospective and based on serologic evidence of infection. These slowly growing and fastidious organisms, however, may be cultured from respiratory samples using special techniques and have been successfully cultured from BAL samples.- Culture has the additional advantage of detecting species other than M pneumoniae, although the clinical significance of such isolates is not clear. Culture, however, is not a method routinely available in most laboratory settings. Recently a DNA probe test for M pneumoniae was made commercially available (Gen-Probe, San Diego, CA). This test has excellent sensitivity and specificity when compared with culture of respiratory secretions. Testing is recommended with pharyngeal swabs but can be used with BAL samples (personal experience). Although no systematic evaluation has been published (to our knowledge) in our hands, approximately 40 percent (6/15) of tests were positive when a request for M pneumoniae probe was made (unpublished data).


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