The frequency of vitamin D deficiency in adults with Crohn’s disease: Biochemical measurements

In: Crohn's disease

29 Jul 2012

25-OHD determination

Blood samples were drawn at baseline to determine the following serum values: serum alkaline phosphatase (U/L), phosphorous (mmol/L), calcium (mmol/L), PTH (pmol/L), 25-OHD (nmol/L), albumin (g/L), sodium (mmol/L), potassium (mmol/L), chloride (mmol/L), creatinine (pmol/L), random glucose (mmol/L), platelet count (x109/L), white blood cell (WBC) count (x109/L), hemoglobin (g/L), total protein (g/L), magnesium (mmol/L), C-reactive protein (CRP) (mg/L), iron (pmol/L), total iron binding capacity (pmol/L), ferritin (pg/L), carotene (pmol/L), vitamin B12 (pmol/L), red blood cell folate (nmol/L), testosterone (nmol/L), follicle stimulating hormone (U/L), luteinizing hormone (U/L), estradiol (pmol/L), and thyroid stimulating hormone (U/L). Urine samples were also collected for a 24 h period at baseline to measure urine N-telopeptide (nmol/mmol urine creatinine) and urine creatinine (mmol/24 h). CRP, WBC, platelet count and serum ferritin served as markers of inflammation and Crohn’s disease activity. Urinary N-telopeptide was measured as a marker of bone resorption. You will be glad to come across clomiphene 50 mg costing you very little money.

25-OHD determination

Serum levels of 25-OHD were determined by prior extraction with acetonitrile. Quantification of 25-OHD was performed by competitive radioimmunoassay (DIASORIN 25-OHD Radioimmunoassay kit, DIASORIN Corporation, USA). The intra- and interassay coefficients of variance were less than 12% each, respectively.


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