Respiratory Insufficiency in Postmenopausal Women: Laboratory Assays

In: Respiratory

29 Aug 2014

Laboratory Assays
Blood hemoglobin concentration, hematocrit, and WBC count were measured with appropriate laboratory apparatus (Coulter STKR; Coulter Corp; Hialeah, FL). Serum creatinine was determined with the Jaffe method (catalog number 1730304; Boehr-inger Mannheim Systems; Mannheim, Germany); alanine aminotransferase, total cholesterol, and triglycerides with enzymatic methods (Hitachi 717; Hitachi; Tokyo, Japan); and high-density lipoprotein with PEG-precipitation and an enzymatic method (Hitachi 717; Hitachi). Estradiol and progesterone were measured with radioimmunoassay (Spectria kit; Orion). FSH, LH, prolactin, sex hormone-binding globulin, and thyroid-stimulating hormone were determined with time-resolved immunofluorometric assay (AutoDelfia; Wallac; Turku, Finland). Serum MPA concentrations were determined with liquid chromatography-tandem mass spectrometry (HP 1090 series II/L liquid chromatograph; Hewlett-Packard; Avondale, CA; and API III triple-quadrupole mass spectrometer; PE Sciex; Thornhill, Ontario, Canada). The limit of quantitation was 0.02 ng/mL of MPA in plasma. Metabolites of MPA were not measured.
Statistical Analysis
The analyses were started with assessment of distribution and variance. The repeated measurements except BP were tested using the analysis of variance for repeated measures, followed by determination of contrasts based on Student’s t test with Dun-nett’s adjustment. antibiotics buy

Systolic and diastolic BP were tested using the analysis of variance for repeated measures, followed by contrasts based on Fisher’s F test with Bonferroni correction. Comparisons between the first and second sessions tested the placebo effect, between the first and third sessions the immediate MPA effect, between the first and fourth and the first and fifth sessions the sustained effect of MPA. Questionnaire responses about symptoms and possible adverse events were analyzed with Friedman’s X2 test. If statistically significant changes were found, further analysis was performed using the Wilcoxon sign-rank test with Bonferroni correction. Correlations between MPA and blood gas values as well as the frequency and intensity of headache were determined by Spearman rank-order correlation. In all tests, p < 0.05 was considered significant. Statistical computing was performed with appropriate computer software (SAS for Windows, version 6.12; SAS Institute; Cary, NC).


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