In order to further understand the processes regulating inflammation and fibrosis in the human lung, we characterized the effects of rIL-1, rTNF, and rIFN-‘Y on human lung fibroblast proliferation and collagen production. In an effort to more accurately simulate the in vivo situation, we analyzed the effects of these cytokines in combination as well as individually. In accord with our previous studies, these investigations demonstrated that rIL-1 and rTNF, individually, stimulate the proliferation and collagen production of quiescent fibroblasts while rIFN-7 stimulates the proliferation without significantly altering the collagen production of the same cells. buy ortho tri-cyclen online
Importantly, these studies also demonstrated that cytokines in combination tend to have less stimulatory, or frankly inhibitory, effects on fibroblast proliferation and collagen production than they have individually. Specifically, in the presence of the factors present in serum, rIFN-7 inhibited fibroblast proliferation and collagen production and rIL-1 and rTNF inhibited fibroblast collagen production. Combining rIFN-7 or rIL-1 with rTNF also resulted in a synergistic inhibition of fibroblast proliferation and diminished collagen production and combining rIL-1 with rIFN-7 negated the stimulatory effects of rIL-1 alone. Lastly, these studies demonstrated that the effects of these cytokine combinations on fibroblast function are mediated by a number of different mechanisms.
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