Clinical response and failure to alter lymphatic function in a guinea pig model (Part 1)

In: Octreotide

3 Aug 2012

guinea pigA well established guinea pig model system was used to assess lymphatic pumping . Guinea pigs (seven to 15 days of age) of either sex were killed by decapitation during halothane anesthesia. Collecting lymphatic vessels (diameter less than 230 pm) supplying the ileum were dissected together with the associated artery and vein, and were left intact within the surrounding mesentery. The tissue was then bathed in a physiological saline solution and the pH was maintained at 7.4 by constant bubbling with 95% 02:5% CO2. The mesentery was used to pin out the tissues on the Sylgard-coated base of a 2 mL organ bath, mounted onto the stage of an inverted microscope (Olympus CK40, Carsen Group Inc, Canada), and lymphatic vessels were continuously superfused at a flow rate of 3 mL/min with the physiological saline solution heated to 36°C. To induce a consistent rate of vessel constrictions, the vessel lumen was perfused through a fine glass cannula inserted into the vessel. The cannula was connected to an infusion pump (KD Scientific, USA) via Teflon tubing and the vessel was perfused in the direction of the valves at a flow rate of 2.5 pL/min. A low-calcium solution (0.3 mM calcium chloride) was used to avoid blocking of the cannula. You have a chance to fight off the disease by taking the exact medicine you need at the exact amount required. Visit the best my canadian pharmacy and find the drug required available at a very appealing price with fast shipping.


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